Have you ever been cutting on a Microtome and had your sections crumble & specimen tear out? Our technicians and experts spent some time brainstorming and rehashing this situation to come up with microtome cutting tips (or, in this case, section preparation tips). Here are some things to watch out for & Simple Solutions to help maintain your sections.
|Insufficient dehydration of Material
||Salvage Rarely Possible|
|Insufficient Clearing of Material
||Return Tissue to Several Changes of Paraffin|
|Incomplete Infiltration with Embedding Medium
||Infiltrate with Paraffin and Re-Embed|
|Extended Time in Paraffin Bath or Bath Too Hot
||Check Thermostat Controls of Paraffin Bath|
|Clearing Fluid Used
||Substitute Chloroform or Toluene instead of Xylene for Clearing|
Each Week Vinnie & our team of Biomedical Technicians, Histologists, & Professionals will sit down to give you Vinnie’s Tip of the Week. Our first series will be on Microtomy Cutting, and simple tips that will save any histotechnologist headaches. We are happy to share our experience.
In return, please follow us on LinkedIn and look around the website.
Until Next Week,
p.s. Feel free to suggest topics or explain issues you have had in our comments below. Our team will review them and incorporate them into future posts.
Past Tips of the Week: